Meat sarcocystosis: a critical meat-borne parasite impacting carcasses in abattoirs
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Keywords
Abstract
One of the most commonly occurring foodborne, tissue cyst-forming protozoan zoonotic parasites with public health and veterinary relevance is Sarcocystis species. This research was conducted using traditional and molecular techniques to identify the incidence of Sarcocystis spp. in 750 slaughtered bovine animals of varying age and sex (375 cattle and 375 buffalo) at various abattoirs in Aswan Governorate, Egypt. The overall occurrence of macroscopic lesions of Sarcocystis spp. in cattle and buffaloes was 20.5% and 38.4%, respectively, while the occurrence of microscopic infection was 30.4% and 70.1%, respectively. Furthermore, the disease strongly correlated with the measured variables, such as the animal’s age and sex. Animals older than 5 years had the highest infection rate, and females had higher infection rates than males. The esophagus, tongue, and diaphragm had the highest rates of sarcocyst compared to other organs. Histopathology studies of sarcocyst in various tissues revealed encased, circular to elongated, basophilic sarcocysts with numerous bradyzoites embedded in muscle fibers. PCR-RFLP reports that S. cruzi, S. hominis, S. hirsuta, S. buffalonis, and S. fusiformis were the species most often found. Moreover, such frequency highlights the pressing need for an effective disease control strategy and a systematic surveillance system among bovine populations. We strongly encourage the One Health approach to reduce zoonotic spread to humans and financial losses in the livestock industry.
Riferimenti bibliografici
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Dalir Ghaffari A., Dalimi A., & Pirestani M. (2022). Microscopic and Molecular Detection of Sarcocystis cruzi (Apicomplexa: Sarcocystidae) in the Heart Muscle of Cattle. Iranian Journal of Parasitology, 17, 535–542. 10.18502/ijpa.v17i4.11281
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Fotouh A., Shosha E. A., Zanaty A. M., & Darwesh M. M. (2024). Immunopathological investigation and genetic evolution of Avian leukosis virus Subgroup-J associated with myelocytomatosis in broiler flocks in Egypt. Virology Journal, 21, 83. 10.1186/s12985-024-02329-7
Gareh A., Soliman M., Saleh A. A., El-Gohary F. A., El-Sherbiny H. M., Mohamed R. H., & Elmahallawy E. K. (2020). Epidemiological and Histopathological Investigation of Sarcocystis spp. in Slaughtered Dromedary Camels (Camelus dromedarius) in Egypt. Veterinary Sciences, 7, 162. 10.3390/vetsci7040162
Gjerde B., Hilali M., & Abbas I. E. (2016). Molecular differentiation of Sarcocystis buffalonis and Sarcocystis levinei in water buffaloes (Bubalus bubalis) from Sarcocystis hirsuta and Sarcocystis cruzi in cattle (Bos taurus). Parasite Research, 115(6), 2459-2471. 10.1007/s00436-016-4998-1
GOVS, 1986. General Organization for Veterinary Services, Law (517).
Gupta A., de Araujo L. S., Hemphill A., Asis K, Benjamin M. R., & Jitender P. D. (2024). Morphological and molecular characterization of a Sarcocystis bovifelis-like sarcocyst in American beef. Parasites Vectors, 17, 543. 10.1186/s13071-024-06628-4
Hajimohammadi B., Dehghani A., Moghadam Ahmadi M., Eslami G., Oryan A., & Khamesipour A. (2014). Prevalence and species identification of Sarcocystis in raw hamburgers distributed in Yazd, Iran using PCR–RFLP. Journal of Food Quality and Hazards Control, 1, 15–20. http://jfqhc.ssu.ac.ir/article-1-47-en.html
Hamidinejat H., Razi Jalali M. H., Gharibi D., & Molayan P. H. (2015). Detection of Sarcocystis spp. in cattle (Bos taurus) and water buffaloes (Bubalus bubalis) in Iran by PCR-RFLP. Journal of Parasitic Diseases, 39, 658–662. 10.1007/s12639-014-0426-6
Hamidinejat H., Jalali M. H., & Nabavi L. (2010). Survey on Sarcocystis infection in slaughtered cattle in South-West of Iran, emphasized on evaluation of muscle squash in comparison with digestion method. Journal of Animal and Veterinary Advances, 9, 1724–1726. 10.3923/javaa.2010.1724.1726
Hoeve-Bakker B. J., van der Giessen J. W., & Franssen F. J. (2019). Molecular identification targeting cox1 and 18S genes confirms the high prevalence of Sarcocystis spp. in cattle in the Netherlands. International Journal for Parasitology, 49, 859–66. 10.1016/j.ijpara.2019.05.008
Hooshyar H., Abbaszadeh Z., Sharafati-Chaleshtori R., & Arbabi M. (2017). Molecular identification of Sarcocystis species in raw hamburgers using PCR-RFLP method in Kashan, central Iran. Journal of Parasitic Diseases, 41, 1001–1005. 10.1007/s12639-017-0925-3
Hornok S., Mester A., Takacs N., Baska F., Majoros G., Fok E., Biksi I., Nemet Z., Hornyak A., Janosi S., & Farkas R. (2015). Sarcocystis infection of cattle in Hungary. Parasites & Vectors, 8, 69–75. 10.1002/9781118649985.ch9
Hussein S. N., Ibrahim A. A., & Shukur M. S. (2023). Histopathology and molecular identification of Sarcocystis species forming macrocysts in slaughtered sheep and goats of Duhok, Iraq. Veterinary Research Forum, 14, 415–422. 10.30466/vrf.2023.559514.3575
Ibrahim M., El Sabagh H., Wahba R. A., & Abd El Rahman, E. S. (2018). The Incidence of Sarcocystis in Slaughtered Food Animals. Benha Veterinary Medicine Journal, 35, 106–122. 10.21608/bvmj.2018.38219
Imre K., Darabus G., Tirziu E., Morariu S., Imre M., Plutzer J., Boldea M. V and Morar A. (2019). Sarcocystis spp. in Romanian slaughtered cattle: Molecular characterization and epidemiological significance of the findings. Biomed Research International, 4123154. 10.1155/2019/4123154
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Apr 1, 2025
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Khairy Elbarbary, N., Bekhit, M. M., Garh, A., Fotouh, A., Dandrawy, M. K., Darwish, W. S., Abdelmotilib, N. M., Ali, M. A., Salem, M. M., & Abdelhaseib, M. (2025). Meat sarcocystosis: a critical meat-borne parasite impacting carcasses in abattoirs. Italian Journal of Food Science, 37(2), 361-373. https://doi.org/10.15586/ijfs.v37i2.2942
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Come citare
Khairy Elbarbary, N., Bekhit, M. M., Garh, A., Fotouh, A., Dandrawy, M. K., Darwish, W. S., Abdelmotilib, N. M., Ali, M. A., Salem, M. M., & Abdelhaseib, M. (2025). Meat sarcocystosis: a critical meat-borne parasite impacting carcasses in abattoirs. Italian Journal of Food Science, 37(2), 361-373. https://doi.org/10.15586/ijfs.v37i2.2942